Device

Part:BBa_K1475008:Design

Designed by: Daniel Weltz Pedersen   Group: iGEM14_SDU-Denmark   (2014-10-05)

lacI protein produced by constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was constructed using PCR to remove the rapid degradation tag from the lacI+LVA registry part (Part:BBa_C0012). In addition to removing the rapid degradation tag, promoter, RBS and terminator was added with the following primers:
Forward (containing: prefix, Pcon:BBa_J23106 and RBS:BBa_B0030):
AAGAATTCGCGGCCGCTTCTAGAGTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCATTAAAGAGGAGAAATACTAGATGAAACCAGTAACGTTATACGATGT
Reverse (containing: suffix and terminator:BBa_B1002):
TTTCTGCAGCGGCCGCTACTAGTAGCGAAAAAACCCCGCCGAAGCGGGGTTTTTTGCGTTATTACTGCCCGCTTTCCAGT



Source

lacI repressor from Escherichia coli

References

UniProt accesstion for Escherichia coli lacI: http://www.uniprot.org/uniprot/P03023
Entrez gene accession for Escherichia coli lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
Protein database entry for Escherichia coli lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG